Prince Frederick Charles of Hesse, King of Finland (1868–1940); married Princess Margaret of Prussia and had issue. Princess Marie-Polyxene of Hesse (1872–1882). Princess Sybille Margaret Adelheid Louise of Hesse (1877–1925); married Friedrich Alexander Henry Robert Carl Albert, Baron von Vincke (divorced 1923).
Prince Wolfgang of Hesse (1896–1989), twin with his brother Philipp. He was the designated Crown Prince of Finland officially until 14 December 1918. He married Princess Marie Alexandra of Baden, no issue; Prince Christoph Ernst August of Hesse (1901–1943), twin with his brother Richard.